dakesan
@dakesan
Public Skills
bio-fasta
by dakesan
"Read/write FASTA, GenBank, FASTQ files. Sequence manipulation (complement, translate). Indexed random access via faidx. For NGS pipelines (SAM/BAM/VCF), use pysam. For BLAST, use gget or blat-integration."
bio-blast
by dakesan
"NCBI BLAST sequence similarity search using BioPython. Use when a user wants to run BLAST programmatically with blastn/blastp and retrieve results in JSON format."
bio-blat
by dakesan
"Runs local BLAT searches for DNA sequence alignment against hg38 or CHM13 using local .2bit references. Use when a user wants to align a DNA sequence without relying on UCSC API access."
bio-bam
by dakesan
"This skill should be used when the user asks to analyze BAM/SAM/CRAM alignment files from WGS/WES sequencing. Triggers include requests to extract reads from specific regions, identify insertions and deletions, calculate coverage statistics, or export read data as JSON for downstream analysis."